6Q6C
Pore-modulating toxins exploit inherent slow inactivation to block K+ channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.835, 64.835, 66.825 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.070 - 1.300 |
| R-factor | 0.16361 |
| Rwork | 0.162 |
| R-free | 0.20248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y62 |
| RMSD bond length | 0.041 |
| RMSD bond angle | 2.217 |
| Data reduction software | Epinorm |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.070 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rpim | 0.061 | 0.714 |
| Number of reflections | 40174 | 3932 |
| <I/σ(I)> | 19.75 | |
| Completeness [%] | 99.4 | 98.84 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.05M Potassium phosphate monobasic and 20% w/v PEG 8,000 |
