6Q20
Crystal structure of human 1E01 Fab in complex with influenza virus neuraminidase from A/Japan/305/1957 (H2N2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 160.452, 160.452, 84.173 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.042 - 2.450 |
R-factor | 0.2201 |
Rwork | 0.217 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tia 5itb 4fql |
RMSD bond length | 0.003 |
RMSD bond angle | 0.665 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.042 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rpim | 0.030 | 0.300 |
Number of reflections | 40758 | 1967 |
<I/σ(I)> | 22.5 | 1.1 |
Completeness [%] | 99.5 | 98.2 |
Redundancy | 13.8 | 10.1 |
CC(1/2) | 0.998 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (v/v) glycerol, 24% (w/v) PEG1500 |