6Q0U
Structure of the Erbin PDZ variant E-6a with a high-affinity C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 34.590, 38.090, 38.450 |
Unit cell angles | 83.04, 74.99, 63.66 |
Refinement procedure
Resolution | 37.140 - 1.890 |
R-factor | 0.184 |
Rwork | 0.178 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Swiss-Modeller model of this PDZ |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.140 | 1.990 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.051 | 0.232 |
Number of reflections | 12494 | 4051 |
<I/σ(I)> | 10.4 | 3.4 |
Completeness [%] | 91.1 | 73.2 |
Redundancy | 2.4 | 2.4 |
CC(1/2) | 0.996 | 0.941 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 14% PEG3350,200 mM MgCl2, 100 mM MES 6.0. Cryoprotected with this condition plus 25% ethylene glycol |