6Q0R

Structure of DDB1-DDA1-DCAF15 complex bound to E7820 and RBM39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	81.108, 93.600, 258.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.050 - 2.900
R-factor	0.207
Rwork	0.205
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fqd chain A
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.030	0.800
Number of reflections	44491	4381
<I/σ(I)>	14.7
Completeness [%]	99.5
Redundancy	6.7
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	20% PEG 4000