6PZF

Crystal structure of human NA-63 Fab in complex with neuraminidase from A/Hunan/02650/2016(H7N9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03319
Spacegroup name	P 4 21 2
Unit cell lengths	164.576, 164.576, 192.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.800 - 2.800
R-factor	0.2055
Rwork	0.203
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l14 5it2 5v7r
RMSD bond length	0.009
RMSD bond angle	1.252
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.850
High resolution limit [Å]	2.800	2.800
Rpim	0.060	0.350
Number of reflections	63525	2555
<I/σ(I)>	10	1
Completeness [%]	96.5
Redundancy	8.4
CC(1/2)	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1 M Tris pH 9.0, 10% glycerol, 10% PEG6000