6PPN
Structure of S. pombe Lsm2-8 with unprocessed U6 snRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 70.280, 135.390, 168.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 168.000 | 2.080 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 80785 | 4039 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 63.3 | |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 289 | 200 mM KCl, 50 mM sodium HEPES pH 7.4, 35 % Pentaerythritol propoxylate (5/4 PO/OH) |