6PKG

Zebrafish N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalytic domain auto-inhibited by pro-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.7712
Spacegroup name	P 41 21 2
Unit cell lengths	124.356, 124.356, 278.792
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.658 - 2.800
R-factor	0.184
Rwork	0.183
R-free	0.20810
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.542
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	90810	4913
<I/σ(I)>	16.1
Completeness [%]	98.9
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	295	50% PEG 200, 0.1M HEPES pH 6.4, soaked in 1M NaI