6PKD
Myocilin OLF mutant N428D/D478H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.547, 47.342, 97.076 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 33.630 - 1.900 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6ou2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.144 | 0.075 | 0.496 |
| Rmeas | 0.166 | 0.087 | 0.584 |
| Rpim | 0.081 | 0.043 | 0.301 |
| Total number of observations | 137258 | ||
| Number of reflections | 33873 | 1780 | 1688 |
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 98.6 | 98.5 | 96.6 |
| Redundancy | 4.1 | 4 | 3.5 |
| CC(1/2) | 0.993 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG 8000, MgCl2 |
