6PH6
Ternary complex crystal structure of DNA polymerase Beta with 2nt-gap with dCTP bound downstream
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-31 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.510, 79.675, 55.428 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 24.929 - 2.600 |
| R-factor | 0.194 |
| Rwork | 0.185 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.075 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.122 | 0.058 | 0.625 |
| Total number of observations | 46342 | ||
| Number of reflections | 12862 | 1301 | 1273 |
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 98.9 | 97.7 | 98.5 |
| Redundancy | 3.6 | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350 |
