6PGL

Structure of Kluyveromyces marxianus Usb1 with uridine monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-17
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	69.957, 110.344, 114.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.172 - 1.845
R-factor	0.1894
Rwork	0.188
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.987
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.520	2.040
High resolution limit [Å]	1.840	1.840
Number of reflections	82782	2827
<I/σ(I)>	15.3
Completeness [%]	94.9
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.2 M potassium sodium tartrate, 100 mM HEPES, 30% glycerol