6PFV
Structure of S. venezuelae RisG-WhiG-c-di-GMP complex: orthorhombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 79.847, 97.339, 204.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.897 - 3.000 |
R-factor | 0.2178 |
Rwork | 0.214 |
R-free | 0.27840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pfj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.975 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.897 | 3.040 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.098 | 0.998 |
Rpim | 0.053 | 0.653 |
Number of reflections | 59828 | 2129 |
<I/σ(I)> | 8.9 | 1.2 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 4 | 3.2 |
CC(1/2) | 0.997 | 0.447 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris Bicine pH 8.5, 0.03 M sodium nitrate, 0.03 M sodium phosphate, 0.03 M ammonium sulfate, 9% MPD, 10% PEG 1000 and 15% PEG 3350 |