6PFV

Structure of S. venezuelae RisG-WhiG-c-di-GMP complex: orthorhombic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	79.847, 97.339, 204.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.897 - 3.000
R-factor	0.2178
Rwork	0.214
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pfj
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.897	3.040
High resolution limit [Å]	2.990	2.990
Rmerge	0.098	0.998
Rpim	0.053	0.653
Number of reflections	59828	2129
<I/σ(I)>	8.9	1.2
Completeness [%]	99.1	99.1
Redundancy	4	3.2
CC(1/2)	0.997	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris Bicine pH 8.5, 0.03 M sodium nitrate, 0.03 M sodium phosphate, 0.03 M ammonium sulfate, 9% MPD, 10% PEG 1000 and 15% PEG 3350