6PFV
Structure of S. venezuelae RisG-WhiG-c-di-GMP complex: orthorhombic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 79.847, 97.339, 204.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.897 - 3.000 |
| R-factor | 0.2178 |
| Rwork | 0.214 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pfj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.975 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.897 | 3.040 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.098 | 0.998 |
| Rpim | 0.053 | 0.653 |
| Number of reflections | 59828 | 2129 |
| <I/σ(I)> | 8.9 | 1.2 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 4 | 3.2 |
| CC(1/2) | 0.997 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris Bicine pH 8.5, 0.03 M sodium nitrate, 0.03 M sodium phosphate, 0.03 M ammonium sulfate, 9% MPD, 10% PEG 1000 and 15% PEG 3350 |
