6PE8
Crystal structure of CD40/ABBV-323 FAB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 173.308, 75.959, 126.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.980 - 2.840 |
R-factor | 0.2081 |
Rwork | 0.205 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pe7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.125 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 173.200 | 2.850 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 40335 | 380 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 | |
CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 296.15 | 2M ammonium sulfate, 0.1M phosphate-citrate pH 4.2 |