6P6D
HUMAN IGG1 FC FRAGMENT, C239 INSERTION MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.192, 81.066, 136.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.310 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26844 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5vgp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.045 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.246 |
| Number of reflections | 24382 | 890 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 94.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 12 mg/mL protein, 20% w/v PEG 3350, 200 mM K Acetate |
