6P50
Crystal Structure of a Complex of human IL-7Ralpha with an anti-IL-7Ralpha Fab 4A10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.113, 40.171, 105.097 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 38.610 - 2.900 |
| R-factor | 0.2879 |
| Rwork | 0.285 |
| R-free | 0.32980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DI3 for IL-7R chain and 6P4Y for 4A10 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.16rc1_3535) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.610 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmeas | 0.283 | 1.140 |
| Number of reflections | 12886 | 1892 |
| <I/σ(I)> | 3.63 | 1.02 |
| Completeness [%] | 96.3 | 89.2 |
| Redundancy | 2.9 | 2.5 |
| CC(1/2) | 0.969 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 25% PEG 3350, 0.2 M sodium malonate pH 6.0, 20% glycerol |
