6P50
Crystal Structure of a Complex of human IL-7Ralpha with an anti-IL-7Ralpha Fab 4A10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.113, 40.171, 105.097 |
Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
Resolution | 38.610 - 2.900 |
R-factor | 0.2879 |
Rwork | 0.285 |
R-free | 0.32980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3DI3 for IL-7R chain and 6P4Y for 4A10 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.505 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | PHENIX (1.16rc1_3535) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.610 | 3.070 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmeas | 0.283 | 1.140 |
Number of reflections | 12886 | 1892 |
<I/σ(I)> | 3.63 | 1.02 |
Completeness [%] | 96.3 | 89.2 |
Redundancy | 2.9 | 2.5 |
CC(1/2) | 0.969 | 0.734 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 25% PEG 3350, 0.2 M sodium malonate pH 6.0, 20% glycerol |