6P4Y
Crystal Structure of anti-IL-7Ralpha 4A10 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03317 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.372, 78.496, 69.480 |
Unit cell angles | 90.00, 96.23, 90.00 |
Refinement procedure
Resolution | 39.248 - 1.799 |
R-factor | 0.1897 |
Rwork | 0.188 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ae6 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15_3448: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.248 | 1.900 |
High resolution limit [Å] | 1.799 | 1.799 |
Rmerge | 0.126 | 1.418 |
Rmeas | 0.149 | 1.418 |
Rpim | 0.080 | 0.921 |
Number of reflections | 36735 | 3613 |
<I/σ(I)> | 8.33 | 0.96 |
Completeness [%] | 99.1 | 96.46 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 0.994 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 30% PEG 5000, 0.1 M sodium acetate, 20% glycerol, seeded |