6P4Y
Crystal Structure of anti-IL-7Ralpha 4A10 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.372, 78.496, 69.480 |
| Unit cell angles | 90.00, 96.23, 90.00 |
Refinement procedure
| Resolution | 39.248 - 1.799 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ae6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15_3448: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.248 | 1.900 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Rmerge | 0.126 | 1.418 |
| Rmeas | 0.149 | 1.418 |
| Rpim | 0.080 | 0.921 |
| Number of reflections | 36735 | 3613 |
| <I/σ(I)> | 8.33 | 0.96 |
| Completeness [%] | 99.1 | 96.46 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.994 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 30% PEG 5000, 0.1 M sodium acetate, 20% glycerol, seeded |
