6P4C
HyHEL10 Fab carrying four heavy chain mutations (HyHEL10-4x): L4F, Y33H, S56N, and Y58F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 68.556, 40.156, 93.649 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 46.470 - 1.850 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9a |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.783 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.470 | 46.470 | 1.890 |
| High resolution limit [Å] | 1.850 | 9.060 | 1.850 |
| Rmerge | 0.051 | 0.027 | 0.862 |
| Rmeas | 0.056 | 0.029 | 0.947 |
| Rpim | 0.022 | 0.012 | 0.386 |
| Total number of observations | 275598 | 2480 | 15523 |
| Number of reflections | 42030 | 403 | 2577 |
| <I/σ(I)> | 16.8 | 50.3 | 2 |
| Completeness [%] | 100.0 | 98.6 | 100 |
| Redundancy | 6.6 | 6.2 | 6 |
| CC(1/2) | 0.999 | 0.999 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM citrate, pH 4.2, 1 M lithium chloride, 11% w/v PEG3350 |
