6OTC
Synthetic Fab bound to Marburg virus VP35 interferon inhibitory domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.827, 97.422, 110.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.960 - 1.700 |
R-factor | 0.1976 |
Rwork | 0.196 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 4GHL & 1TZI |
RMSD bond length | 0.006 |
RMSD bond angle | 1.307 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.960 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 68713 | 3348 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.4 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 10 mM Bis-Tris, pH 6.5, 20% PEG5000 MME |