6OQ5
Structure of the full-length Clostridium difficile toxin B in complex with 3 VHHs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 149.619, 168.565, 179.921 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.910 - 3.870 |
R-factor | 0.26541 |
Rwork | 0.263 |
R-free | 0.31516 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bvl 4r04 3pee 6c0b 3v0a 4np4 and 4NC2 |
RMSD bond length | 0.003 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 3.970 |
High resolution limit [Å] | 3.870 | 3.870 |
Rmerge | 0.137 | 1.548 |
Number of reflections | 42971 | 2123 |
<I/σ(I)> | 7.7 | |
Completeness [%] | 99.4 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium acetate, 0.1M magnesium acetate, and 5% PEG 8K (final pH 5.2) |