6OQ4
Crystal Structure of the Ternary Complex of KRIT1 bound to both the Rap1 GTPase and HKi1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.350, 77.410, 58.620 |
Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
Resolution | 58.596 - 1.754 |
Rwork | 0.227 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 1.146 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.600 | 1.860 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.042 | 0.911 |
Number of reflections | 51111 | 8192 |
<I/σ(I)> | 17.84 | |
Completeness [%] | 99.5 | |
Redundancy | 4.1 | |
CC(1/2) | 0.999 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20-25% PEG 3,350, 100 mM Tris, pH 8.5, 100 mM KCl |