6OQ4
Crystal Structure of the Ternary Complex of KRIT1 bound to both the Rap1 GTPase and HKi1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.350, 77.410, 58.620 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 58.596 - 1.754 |
| Rwork | 0.227 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.600 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.042 | 0.911 |
| Number of reflections | 51111 | 8192 |
| <I/σ(I)> | 17.84 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.999 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20-25% PEG 3,350, 100 mM Tris, pH 8.5, 100 mM KCl |
