6OPA

Crystal structure of bovine Fab NC-Cow1 in complex with HIV-1 BG505 SOSIP.664, and human Fabs 35022 and PGT128

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	H 3 2
Unit cell lengths	215.244, 215.244, 438.798
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.755 - 4.084
R-factor	0.3328
Rwork	0.332
R-free	0.34310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez 4toy 3tv3 6oo0
RMSD bond length	0.004
RMSD bond angle	0.940
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.900	4.170
High resolution limit [Å]	4.080	4.080
Rmeas	0.232	2.280
Rpim	0.076	0.760
Number of reflections	30902	1515
<I/σ(I)>	11.2	0.34
Completeness [%]	100.0	100
Redundancy	9.2	8.9
CC(1/2)	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.56	298	0.2M sodium citrate, 0.1M Tris, 30% Peg400