EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 4.4 |
Number of Particle | 75909 |
Software | EMAN2,SPIDER,Coot,PHENIX,CTFFIND,RosettaEM |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |