6OF1

Crystal structure of the Thermus thermophilus 70S ribosome in complex with dirithromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.80A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	208.150, 446.400, 615.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	253.382 - 2.800
R-factor	0.217
Rwork	0.215
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.846
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	253.382	253.382	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.279	0.035	3.093
Rmeas	0.294	0.037	3.262
Total number of observations	14260035
Number of reflections	1386444	16398	101531
<I/σ(I)>	10.18	58.37	0.93
Completeness [%]	99.8	99.5	99.3
Redundancy	10.285	9.473	9.852
CC(1/2)	0.996	0.999	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME