Crystal structure of the Thermus thermophilus 70S ribosome in complex with dirithromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.80A resolution

This is a large structure.

Summary for 6OF1

Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (61 entities in total)
Functional Keywordsdirithromycin, macrolide, antibiotic, 70s ribosome, inhibitor, nascent peptide exit tunnel, ribosomal protein ul4, ribosome
Biological sourceEscherichia coli
Total number of polymer chains112
Total molecular weight4573555.65
Primary citation
Khabibullina, N.F.,Tereshchenkov, A.G.,Komarova, E.S.,Syroegin, E.A.,Shiriaev, D.I.,Paleskava, A.,Kartsev, V.G.,Bogdanov, A.A.,Konevega, A.L.,Dontsova, O.A.,Sergiev, P.V.,Osterman, I.A.,Polikanov, Y.S.
Structure of dirithromycin bound to the bacterial ribosome suggests new ways for rational improvement of macrolides.
Antimicrob. Agents Chemother., 2019
PubMed: 30936109 (PDB entries with the same primary citation)
DOI: 10.1128/AAC.02266-18
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.2639 0.9% 5.6% 5.4% 0.53MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6of1
no rotation
Molmil generated image of 6of1
rotated about x axis by 90°
Molmil generated image of 6of1
rotated about y axis by 90°