6OAZ
Apo Structure of WT Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D produced in HEK293-F cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.000, 191.940, 96.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.520 - 3.040 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lpa 2j8b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 191.940 | 191.940 | 3.200 |
| High resolution limit [Å] | 3.040 | 9.610 | 3.040 |
| Rmerge | 0.105 | 0.042 | 0.922 |
| Rmeas | 0.117 | 0.046 | 1.017 |
| Rpim | 0.049 | 0.020 | 0.422 |
| Total number of observations | 303821 | 9638 | 47721 |
| Number of reflections | 55090 | 1954 | 8190 |
| <I/σ(I)> | 10.6 | 30.6 | 2.2 |
| Completeness [%] | 97.2 | 99 | 99.9 |
| Redundancy | 5.5 | 4.9 | 5.8 |
| CC(1/2) | 0.997 | 0.997 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.15M calcium acetate, 18% PEG3350 |
