6OAU
Apo Structure of WT Lipoprotein Lipase in Complex with GPIHBP1 Mutant N78D N82D produced in GnTI-deficient HEK293-F cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.480, 97.250, 77.210 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 35.500 - 2.480 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lpa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER (2.11.7) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 88.580 | 88.580 | 2.620 |
| High resolution limit [Å] | 2.480 | 7.850 | 2.480 |
| Rmerge | 0.125 | 0.105 | 0.546 |
| Rmeas | 0.148 | 0.126 | 0.651 |
| Rpim | 0.080 | 0.069 | 0.352 |
| Total number of observations | 154530 | ||
| Number of reflections | 45839 | 1459 | 6706 |
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 98.8 | 95.1 | 99.9 |
| Redundancy | 3.4 | 3.2 | 3.3 |
| CC(1/2) | 0.978 | 0.963 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium Malonate, 20% PEG3350, 4% 2-propanol |
