6OAR

Structure of the Kupe virus OTU bound to the C-terminal domain of sheep ISG15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9200
Spacegroup name	P 21 21 21
Unit cell lengths	41.796, 158.758, 171.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.289 - 2.063
R-factor	0.1785
Rwork	0.176
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	composite homology model generated from PDB entries 4HXD 5jze 3pse and 3PHX
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.060	2.060
Number of reflections	70403	6137
<I/σ(I)>	15.9	1.51
Completeness [%]	99.8	99.9
Redundancy	5.1	4.6
CC(1/2)	0.994	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium acetate pH 4.1, 2.2 M potassium acetate