6O3D
Crystal structure of the unbound Fab fragment of the human HIV-1 neutralizing antibody PGZL1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.590, 96.021, 67.325 |
Unit cell angles | 90.00, 120.18, 90.00 |
Refinement procedure
Resolution | 37.035 - 1.402 |
R-factor | 0.1481 |
Rwork | 0.146 |
R-free | 0.18120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fx7 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.391 |
Data reduction software | HKL-2000 (2.3.10) |
Data scaling software | HKL-2000 (2.3.10) |
Phasing software | PHASER (2.3.10) |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.048 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rpim | 0.052 | 0.609 |
Number of reflections | 89730 | 3906 |
<I/σ(I)> | 7.8 | 1.4 |
Completeness [%] | 95.4 | 83.2 |
Redundancy | 3.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | Reservoir: 20% PEG 4000, 20% glycerol, 0.08 M sodium acetate pH 4.6, 0.16 M ammonium sulfate |