6O39

Crystal structure of Frizzled 5 CRD in complex with F2.I Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-14
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 61 2 2
Unit cell lengths	101.405, 101.405, 196.497
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.472 - 1.800
R-factor	0.1976
Rwork	0.196
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f0a
RMSD bond length	0.006
RMSD bond angle	0.881
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.480	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	56003
<I/σ(I)>	25.13
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293