6O1F
Complex between soybean trypsin inhibitor beta1-tryptase and a humanized fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2015-04-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97395 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 128.520, 128.520, 245.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.480 - 2.150 |
| R-factor | 0.1911 |
| Rwork | 0.190 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvc 4a6l 1avu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.480 | 2.226 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.086 | 0.681 |
| Number of reflections | 65608 | 6404 |
| <I/σ(I)> | 14.2 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 5% PEG 4000 0.1M lithium sulfate 0.1M Tris pH 7.5 |
