6O0T
Crystal structure of selenomethionine labelled tandem SAM domains (L446M:L505M:L523M mutant) from human SARM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 94.240, 154.080, 120.995 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 43.190 - 2.800 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.507 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((DEV_3357: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.490 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.152 | 1.185 |
| Rmeas | 0.182 | 1.412 |
| Rpim | 0.099 | 0.760 |
| Number of reflections | 41934 | 4410 |
| <I/σ(I)> | 6.8 | 1.3 |
| Completeness [%] | 99.0 | 96.9 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.988 | 0.431 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 17% PEG 8000, 20% ethylene glycol, 0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M trisodium citrate, 0.2 M sodium potassium L-tartrate, 10 mM DTT and 0.1 M Tris pH 7.9 |
