6O03
Monobody (MC17) bound to tyrosine kinase binding domain of E3 ubiquitin ligase CBL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-30 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.104, 67.156, 167.882 |
Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
Resolution | 46.190 - 3.300 |
R-factor | 0.2891 |
Rwork | 0.285 |
R-free | 0.32970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.802 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.490 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 14407 | |
<I/σ(I)> | 8.66 | |
Completeness [%] | 98.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 3000, 100mM tris base/hydrochloric acid pH 7, 200mM calcium acetate |