6NQ3

Crystal Structure of a SUZ12-RBBP4-PHF19-JARID2 Heterotetrameric Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-06-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	127.710, 139.600, 268.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.600 - 2.890
R-factor	0.173
Rwork	0.170
R-free	0.23000
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.110	2.950
High resolution limit [Å]	2.890	2.890
Number of reflections	52773
<I/σ(I)>	17.3
Completeness [%]	98.9
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% polyethylene glycol (PEG) 3350, 0.1M Sodium Malonate, pH6.0