6NN7

The structure of human liver pyruvate kinase, hLPYK-GGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	79.365, 106.205, 151.711
Unit cell angles	76.44, 80.06, 71.37

Refinement procedure

Resolution	39.060 - 2.320
R-factor	0.192
Rwork	0.191
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ip7
RMSD bond length	0.012
RMSD bond angle	1.252
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.060	2.360
High resolution limit [Å]	2.320	2.320
Number of reflections	175876
<I/σ(I)>	12.3
Completeness [%]	89.4
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.2 M ammonium citrate, pH 6.0, 24% PEG3350