6NN5

The structure of human liver pyruvate kinase, hLPYK-W527H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	120.010, 204.624, 112.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.182 - 2.256
R-factor	0.2274
Rwork	0.225
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ip7
RMSD bond length	0.012
RMSD bond angle	1.268
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.312	2.327
High resolution limit [Å]	2.256	2.256
Number of reflections	84688
<I/σ(I)>	14.9
Completeness [%]	90.4
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.325 M ammonium citrate dibasic, 16% PEG3350