6NMW

Crystal structure of the human Lyn SH3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	105
Detector technology	PIXEL
Collection date	2018-07-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 61
Unit cell lengths	46.722, 46.722, 55.696
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.736 - 1.199
R-factor	0.173
Rwork	0.172
R-free	0.18270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iim
RMSD bond length	0.014
RMSD bond angle	1.602
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.199	1.200
Rpim	0.012	0.171
Number of reflections	18850	12664
<I/σ(I)>	3.55
Completeness [%]	87.1	81.8
Redundancy	5.7
CC(1/2)	0.994	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Na citrate, 3.2 M NaCl