6NK0
EphA2 LBD in complex with bA-WLA-Yam peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-14 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.798, 78.060, 58.340 |
| Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
| Resolution | 28.830 - 1.530 |
| R-factor | 0.1525 |
| Rwork | 0.151 |
| R-free | 0.17710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hei |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.319 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14rc2-3191) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.830 | 1.550 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.040 | 0.463 |
| Rmeas | 0.047 | 0.581 |
| Rpim | 0.024 | 0.344 |
| Number of reflections | 61846 | 2631 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 98.2 | 85 |
| Redundancy | 3.6 | 2.7 |
| CC(1/2) | 0.999 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.09M BIS-TRIS pH5.5, 22.5% w/v PEG 3,350, 3% w/v 6-aminohexanoic acid |
