6NJT
Mouse endonuclease G mutant - H97A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-06-17 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.609, 109.609, 118.062 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.710 - 2.070 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gkp |
Data reduction software | XDS |
Data scaling software | Aimless (v0.7.1) |
Phasing software | PHASER (v2.7.16) |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.800 | 2.130 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.277 | 2.691 |
Rpim | 0.145 | 1.618 |
Number of reflections | 50410 | 3865 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.8 | 7.1 |
CC(1/2) | 0.992 | 0.319 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 290 | 25% isopropanol, 0.2 M MgCl2, 0.1 M HEPES pH 7.6, 0.75 mM G(5HMC) dinucleotide |