6NFJ
Structure of Beta-Klotho in Complex with FGF19 C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.090, 143.900, 141.050 |
Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
Resolution | 59.420 - 3.190 |
R-factor | 0.2815 |
Rwork | 0.280 |
R-free | 0.31960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vaq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.446 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.420 | 3.310 |
High resolution limit [Å] | 3.190 | 3.200 |
Number of reflections | 42869 | |
<I/σ(I)> | 3.4 | 0.81 |
Completeness [%] | 99.2 | 95.87 |
Redundancy | 3.8 | 3.3 |
CC(1/2) | 0.519 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 21% PEG1500, 0.1M Ammonium sulphate, 0.2M NDSB-201, 0.1M Tris-HCl pH 7.5 |