6ND6

Crystal structure of the Thermus thermophilus 70S ribosome in complex with erythromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.85A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	210.050, 448.090, 621.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.045 - 2.850
R-factor	0.2522
Rwork	0.249
R-free	0.30470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.003
RMSD bond angle	0.672
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.045	224.045	2.920
High resolution limit [Å]	2.850	12.750	2.850
Rmerge	0.188	0.019	1.375
Rmeas	0.224	0.023	1.656
Total number of observations	4480046
Number of reflections	1330642	15232	96222
<I/σ(I)>	6.8	35.65	0.85
Completeness [%]	98.8	95.3	97
Redundancy	3.367	3.296	3.109
CC(1/2)	0.989	0.999	0.122

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME