6NCM
Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead-like (FHL) DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.540, 72.310, 102.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.300 - 2.704 |
R-factor | 0.2377 |
Rwork | 0.234 |
R-free | 0.27550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nce |
RMSD bond length | 0.003 |
RMSD bond angle | 0.484 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.300 | 2.801 |
High resolution limit [Å] | 2.704 | 2.704 |
Rmerge | 0.090 | 1.949 |
Rmeas | 0.099 | 2.115 |
Rpim | 0.039 | |
Number of reflections | 9108 | 873 |
<I/σ(I)> | 11.66 | 0.85 |
Completeness [%] | 99.2 | 98.87 |
Redundancy | 6.5 | 6.6 |
CC(1/2) | 0.999 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 0.1 M BisTris pH 5.5, 0.2 M NaCl, 22% PEG 3350 |