6NAZ
Crystal structure of DIRAS 2/3 chimera in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.030, 92.030, 86.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.700 - 3.081 |
| R-factor | 0.2701 |
| Rwork | 0.267 |
| R-free | 0.33170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.618 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.700 | 3.190 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Number of reflections | 4324 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1500 mM Ammonium sulfate, 100 mM Tris base/Hydrochloric acid pH 8.5, 12% glycerol |
