6N69
rat hPGDS complexed with a quinoline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-12 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.188, 80.997, 97.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.290 - 2.000 |
| R-factor | 0.18504 |
| Rwork | 0.182 |
| R-free | 0.23145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.341 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.290 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.405 |
| Number of reflections | 29490 | 2022 |
| <I/σ(I)> | 9.5 | 2.7 |
| Completeness [%] | 99.3 | 94.3 |
| Redundancy | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.2M NaF, 24.0%w/v PEG 3350 |
