6N46

Crystal structure of the cryptic polo box domain of a human activated Plk4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-11
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9793
Spacegroup name	P 32
Unit cell lengths	110.831, 110.831, 205.682
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.810 - 3.708
R-factor	0.2734
Rwork	0.271
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n9j
RMSD bond length	0.005
RMSD bond angle	0.868
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3235)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.830
High resolution limit [Å]	3.700	3.700
Number of reflections	29891
<I/σ(I)>	23.75	2
Completeness [%]	100.0	100
Redundancy	11	9.7
CC(1/2)		0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	281	22.5% (w/v) PEG2000 3% (w/v) dextran sulfate sodium salt 0.1M bicine (pH 8.5) 24 uM nonaethylene glycol monododecyl ether (C12E9) 8% (w/v) glycerol