6N45

Crystal structure of the cryptic polo box domain of human activated Plk4 variant 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-03
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9793
Spacegroup name	P 32
Unit cell lengths	61.659, 61.659, 137.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.770 - 2.640
R-factor	0.23803
Rwork	0.234
R-free	0.31396
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n9j
RMSD bond length	0.012
RMSD bond angle	2.005
Data reduction software	HKL-2000 (715.5)
Data scaling software	HKL-2000 (715.5)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.730
High resolution limit [Å]	2.640	2.640
Number of reflections	17096
<I/σ(I)>	49.7	2.8
Completeness [%]	99.9	100
Redundancy	11.7	11.6
CC(1/2)		0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	4.3M Ammonium Acetate 0.1M BisTris propane pH 8.0