6N3P
Crosslinked AcpP=FabZ complex from E. coli Type II FAS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.136, 136.185, 152.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.495 - 2.500 |
R-factor | 0.2115 |
Rwork | 0.210 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1z |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.490 | 49.490 | 2.480 |
High resolution limit [Å] | 2.410 | 9.920 | 2.410 |
Rmerge | 0.218 | 0.073 | 2.703 |
Rmeas | 0.238 | 0.079 | 2.933 |
Rpim | 0.093 | 0.032 | 1.130 |
Total number of observations | 344635 | 4819 | 30145 |
Number of reflections | 53399 | 873 | 4487 |
<I/σ(I)> | 6.8 | 17.6 | 1.3 |
Completeness [%] | 99.8 | 98.9 | 98 |
Redundancy | 6.5 | 5.5 | 6.7 |
CC(1/2) | 0.992 | 0.996 | 0.303 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Bis-Tris pH 6.5, 200 mM MgCl2, 19% PEG 3350, 1:1 mix of 9.5 mg/mL protein and reservoir solution. |