6MZW
TAS-120 covalent complex with FGFR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.919, 48.981, 65.119 |
| Unit cell angles | 90.00, 106.19, 90.00 |
Refinement procedure
| Resolution | 47.630 - 2.200 |
| R-factor | 0.24379 |
| Rwork | 0.241 |
| R-free | 0.29695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ur1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 32531 | 2792 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.999 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MPEG 5000, ammonium sulfate, sodium cacodylate |
