6MXO

Structure of HPoleta incorporating dCTP opposite the 3-prime Pt(DACH)-GG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 61
Unit cell lengths	98.560, 98.560, 81.713
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.860 - 2.040
R-factor	0.17744
Rwork	0.175
R-free	0.22999
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dl2
RMSD bond length	0.018
RMSD bond angle	1.898
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.040	2.040
Rmerge	0.133
Number of reflections	28856
<I/σ(I)>	19.54	3
Completeness [%]	99.2	98.3
Redundancy	11.3	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M MES, 5mM MgCl2, 15-20%PEG2K-MME