6MXG
Crystal structure of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltranferase in complex with XMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.570, 94.370, 45.000 |
| Unit cell angles | 90.00, 111.97, 90.00 |
Refinement procedure
| Resolution | 47.185 - 2.392 |
| R-factor | 0.2613 |
| Rwork | 0.257 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jsq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.621 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.350 | 2.520 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.125 | 0.800 |
| Rpim | 0.060 | 0.397 |
| Number of reflections | 17999 | 2577 |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 99.6 | 97.4 |
| Redundancy | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 25% PEG 3350, 0.2 M lithium sulfate and 0.1 M Bis-Tris |
