6MWG

NavAb Voltage-gated Sodium Channel, residues 1-239, with mutation T206V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	124.175, 124.175, 189.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.501
R-factor	0.2211
Rwork	0.220
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.007
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	25875
<I/σ(I)>	24.6
Completeness [%]	99.8
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.8