6MVY

NavAb voltage-gated sodium channel, residues 1-226, crystallized in the presence of Class 1B Anti-arrhythmic drug Lidocaine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	126.596, 126.596, 192.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.033 - 3.002
R-factor	0.2055
Rwork	0.204
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.007
RMSD bond angle	0.880
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.109
High resolution limit [Å]	3.000	3.002
Rmerge	0.120
Number of reflections	30052
<I/σ(I)>	24
Completeness [%]	100.0
Redundancy	16
CC(1/2)		0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.8 M Ammonium Sulfate 100 mM Sodium Acetate, pH 5.0 10 mM Lidocaine